African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6638

Full Length Research Paper

Evaluation of some bacterial isolates as germination stimulants of Striga hermonthica

O. O. Babalola1*, D. K. Berner2 and N. A. Amusa3
1Department of Microbiology, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria  2Plant Health Management Division, International Institute of Tropical Agriculture, Ibadan, Nigeria. Present address: United States Department of Agriculture, Agricultural Research Service, Foreign Disease-Weed Science Research Unit, Frederick, MD 21702, USA.  3Department of Plant Science and Applied Zoology, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
Email: [email protected]

  •  Accepted: 29 December 2006
  •  Published: 31 January 2007

Abstract

To investigate the role of Pseudomonas sp., Klebsiella oxytoca and Enterobacter sakazakii in Striga 
hermonthica seed germination, we have used two varieties of sorghum to test over time for the selected 
bacteria, using GR-24, a synthetic strigol analogue as a standard and water as a check. Sorghum seeds 
coated with a mixture of bacterial cells (5 x 107cfu ml-1) from 24 h old cultures were planted in 
pasteurized potted soil, which was infested with 0.05 g (about 3000) viable S. hermonthica seed per pot 
14 d before sowing. Results have indicated that the bacterial isolates could stimulate S. hermonthica 
germination in the laboratory and in the screen-house. In vitro, only Pseudomonas sp. 4MKS8 gave 
significant stimulation of S. hermonthica seed at 5% level of probability as compared to the water 
check. In the screen-house, the four bacterial isolates stimulated significant germination of S. 
hermonthica. 
 
Key words: Striga hermonthica, Pseudomonas sp., Klebsiella oxytoca, Enterobacter sakazakii, germination 
stimulant, sorghum.