Full Length Research Paper
Abstract
In this study, we analyzed the effect of different carbon sources on: (i) carbohydrate and nutrient absorption in Chinese Ash (Fraxinus mandshurica) explants in vitro, and (ii) explant survival rate during ex vitroacclimatization. The explants were cultured under one of five conditions: (1) in a solid medium supplemented with 30 g l-1 sucrose (SM); (2) in a modified temporary immersion bioreactor (TIB) supplemented with sucrose at 0 (TIB0) or (3) 30 g l-1 (TIBS) concentration; (4) in TIB flushed with CO2 at 700 to 900 µl l-1 (TIBC) with no sucrose; or in TIB flushed with CO2 at 700 to 900 µl/l while simultaneously adding sucrose 30 g l-1 (TIBCS). The explants cultured under TIBCS exhibited the highest level of root induction and acclimatization survival rate. In addition, these explants exhibited maximized fresh weights (FW), dry weights (DW), and sucrose contents. Explants cultured in SM showed the highest rate of carbohydrate accumulation. At the end of the culture incubation period, absorption of nitrogen, phosphorus, potassium, magnesium, iron, zinc and manganese was also higher in explants cultured in SM than in TIB. This study revealed that the use of TIBCS provided optimum explant survival.
Key words: Fraxinus mandshurica, temporary immersion bioreactor, CO2 enrichment, nutrient absorption. |
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