African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6691

Full Length Research Paper

Humic acids and brassinosteroid application effects on pineapple plantlet growth and nutrition during the aclimatization phase

Paulo Cesar dos Santos
  • Paulo Cesar dos Santos
  • Setor de Horticultura, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Darcy Ribeiro‎, Brazil.
  • Google Scholar
Almy Junior Cordeiro de Carvalho
  • Almy Junior Cordeiro de Carvalho
  • Setor de Horticultura, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Darcy Ribeiro‎, Brazil.
  • Google Scholar
Mírian Peixoto Soares da Silva
  • Mírian Peixoto Soares da Silva
  • Instituto Federal do Tocantins (IFTO) Campus Avançado Pedro Afonso, Brazil.
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Diego Alves Pecanha
  • Diego Alves Pecanha
  • Setor de Nutrição Mineral de Plantas, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Darcy Ribeiro‎, Brazil.
  • Google Scholar
Aurilena de Aviz Silva
  • Aurilena de Aviz Silva
  • Setor de Horticultura, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Darcy Ribeiro‎, Brazil.
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Tiago Massi Ferraz
  • Tiago Massi Ferraz
  • Centro de Ciências Agrárias, Universidade Estadual do Maranhão (UEMA), Brazil.
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Marta Simone Mendonca Freitas
  • Marta Simone Mendonca Freitas
  • Setor de Horticultura, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Darcy Ribeiro‎, Brazil.
  • Google Scholar


  •  Received: 15 May 2018
  •  Accepted: 11 June 2018
  •  Published: 26 July 2018

Abstract

Humic acid and brassinosteroid applications may be an alternative to decrease the pineapple plantlet acclimatization in in vitro cultivation, since promising results have been observed when these substances were independently applied in other propagation methods. In this sense, the aim of the present study was to evaluate the effects of humic acids and brassinosteroid application on 'BRS Vitória' pineapple plantlets grown from in vitro cultivation during acclimatization. A randomized block design was used in a 5x2x4 factorial scheme, at five brassinosteroid doses (0, 0.25, 0.50, 0.75, 1.0 mg L-1) in the presence and absence of humic acids during four sampling periods (60, 90, 120 and 150 days after transplanting), with five replicates for each treatment. BIOBRÁS-16 was used as the brassinosteroid source, and the organic soil conditioner Agrolmin HF® was used as the humic acid source. Plantlets were collected for evaluation every 30 days from 60 days after transplanting. The number of plantlet leaves, length and root mass were higher in the humic acid treatment without brassinosteroid application. Leaf, nitrogen, phosphorus, potassium, calcium and magnesium contents were of 13.04, 1.77, 40.2, 8.79 and 3.17 mg kg-1, respectively. Nitrogen and potassium contents in the plantlets decreased, while phosphorus contents increased as a function of acclimatization time, regardless of treatment.

Key words: Ananas comosus var. comosus, propagation, in vitro.