African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Scanning electron microscopy-energy dispersive X-ray spectrometer (SEM-EDX) detection of arsenic and cadmium in himematsutake mushroom

Chun-Yen Liu
  • Chun-Yen Liu
  • Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.
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Chao-Jen Wang
  • Chao-Jen Wang
  • Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.
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Wen-Chuan Chung
  • Wen-Chuan Chung
  • Taiwan Seed Improvement and Propagation Station, Hsinhse, Taichung 426, Taiwan.
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Chien Chu
  • Chien Chu
  • Taiwan Agricultural Chemicals and Toxic Substances Research Institute Council of Agriculture, Executive Yuan, Taichung 413, Taiwan.
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Wan-Yu Lin
  • Wan-Yu Lin
  • Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.
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Wen-Hsin Chung*
  • Wen-Hsin Chung*
  • Department of Plant Pathology, National Chung Hsing University, Taichung 40227, Taiwan.
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  •  Received: 16 October 2014
  •  Accepted: 23 February 2015
  •  Published: 11 March 2015

Abstract

The distribution of arsenic (As) and cadmium (Cd) in himematsutake was analyzed using scanning electron microscopy-energy dispersive X-ray spectrometer (SEM-EDX). The atomic percentage of the metals was confirmed by inductively coupled plasma-mass spectrometer (ICP-MS). Results show that the accumulation of As in pileus was higher than that in stipe by both SEM-EDX and ICP-MS analyses. The Cd level in pileus was higher than that in stipe by SEM-EDX analysis; whereas a higher level of Cd was found in stipe by ICP-MS analysis. Results of regression analysis showed that there was a positive correlation between the content of As and Cd in the piece samples by both SEM-EDX and ICP-MS methods. Results suggest that the SEM-EDX is one of the potential tools for rapid detection of metals, namely, As and Cd in himematsutake.

 

Key words: Arsenic (As), cadmium (Cd), scanning electron microscopy-energy dispersive X-ray spectrometer (SEM-EDX), coupled plasma-mass spectrometer (ICP-MS), himematsutake. 

Abbreviation

SEM-EDX, Scanning electron microscopy-energy dispersive X-ray spectrometer; As, arsenic; Cd, cadmium; ICP-MS, inductively coupled plasma-mass spectrometer; AAS, atomic absorption spectrometry. 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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