African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Studying arsenic trioxide-induced apoptosis of Colo-16 cells with two-photon and confocal laser scanning microscopy

Hu Jian-jiang1, Zhang Qian2, Hou Yan-ming1, Wang Xin-rui3, Ma Hui4 and  Wang Yi1*
1Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China. 2School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China. 3Foreign Language Department, China Pharmaceutical University, Nanjing 210009, China. 4Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Email: [email protected]

  •  Accepted: 12 May 2011
  •  Published: 05 October 2011

Abstract

With two-photon and confocal laser scanning microscopy in combination with fluorescent probes Hoechst 33342, 2¢,7¢-dichlorofluorescin diacetate (DCFH-DA) and Fluo 3-AM, we simultaneously observed arsenic trioxide (As2O3)-induced changes in nuclear morphology, reactive oxygen species (ROS) and intracellular calcium concentration [Ca2+]i within human skin squamous carcinoma cells (Colo-16 cells). Our results indicated that As2O3 induced [Ca2+]i elevation and ROS production within Colo-16 cells, and both [Ca2+]i elevation and ROS production were involved in the apoptosis of Colo-16 cells. These results suggested that two-photon and confocal laser scanning microscopy might provide a real-time, sensitive and noninvasive method for simultaneously multi-parameter observation of As2O3-induced apoptosis at the single cell level.

 

Key words: Two-photon laser scanning microscopy, confocal laser scanning microscopy, human skin squamous carcinoma cells (Colo-16 cells), arsenic trioxide, apoptosis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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