African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular and pomological diversity among pomegranate (Punica granatum L.) cultivars in Eastern Mediterranean region of Turkey

CoÅŸkun Durgaç1, Mustafa Özgen2, Özhan ÅžimÅŸek3, Yıldız Aka Kaçar3,4, Yelda Kıyga1, Semih Çelebi1, Kazim Gündüz1 and Sedat Serçe1*
  1Mustafa Kemal University, Faculty of Agriculture, Department of Horticulture, Antakya, Hatay, Turkey, 31034. 2GaziosmanpaÅŸa University, Faculty of Agriculture, Department of Horticulture, TaÅŸlıçiftlik, Tokat, Turkey, 60250. 3Çukurova University, Institute of Basic and Applied Science, Biotechnology Department, Adana, Turkey, 01030. 4Çukurova University, Faculty of Agriculture, Department of Horticulture, Adana, Turkey, 01030.
Email: [email protected]

  •  Accepted: 02 April 2008
  •  Published: 02 May 2008

Abstract

 

Pomegranate (Punica granatum L.) is an important fruit species for Turkey where many cultivars are being cultivated. In this study, we determined the fruit characteristics and RAPD band patterns of six local cultivars from Hatay, Turkey. Our results demonstrated that there is a great level of morphological variation. The principle component analysis of 18 quantitative fruit characteristics revealed that fruit weight, aril number/fruit, peel color and soluble solids/acidity ratio are important traits for discriminating the cultivars tested. The UPGMA cluster of fruit characteristics indicated that ‘Katırbaşı’ and ‘Kan narı’ were similar to each other and they were separated from rest of the cultivars. Twenty-two RAPD primers generated total of 106 reproducible bands 22% of which were polymorphic. The UPGMA dendrogram of RAPD data showed that ‘Tatlı nar’ and ‘Åžerife’ were very closely related while ‘Ä°ncekabuk’ is distinct from the other cultivars. As a result, discrepancies were detected between morphological and molecular data. Therefore, we confirmed that diversity among the fruit characteristics were not good indication of genetic relatedness while molecular tools are valuable to study such similarities.

 

Key words: Genetic resource, genetic variability, pomegranate, Punica granatum, RAPD.