Pathogenicity and proteome production of Isaria fumosorosea (=Paecilomyces Fumosoroseus) (WISE) isolates against lemon butterfly, Papilio demoleus (Papilionidae: lepidoptera)

Pasco B. Avery
University of Florida, Institute of Food and Agricultural Sciences, Indian River Research and Education Centre. 2199 South Rock Road, Fort Pierce, FL34945, USA.
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Selvarani, S.

Selvarani, S.
Department of Zoology, Thiagarajar College, Madurai-625009, India.
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T. Kubendran

T. Kubendran
Department of Zoology, Thiagarajar College, Madurai-625009, India.
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T. Rathinakumar

T. Rathinakumar
Department of Zoology, Thiagarajar College, Madurai-625009, India.
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Article Number - 1D07FA548206
Vol.13(43), pp. 4176-4182 , October 2014
https://doi.org/10.5897/AJB12.641

Received: 22 February 2012
Accepted: 01 June 2012
Published: 22 October 2014

Abstract

The pathogenic potential and catalytic triad conserved amino acids of the isolates Isaria fumosorosea (=Paecilomyces fumosoroseus) (Ifr₁ and Ifr₂) in response to Papilio demoleus was analysed. The isolates showed its potential in killing P. demoleus causing mortality of 72.23 and 61.90% at the end of 8 days with 10⁸ spores ml^-1 concentrations. The enzyme assays (higher proteolytic and chitinolytic activity) also showed that the Ifr₂ was more efficient than Ifr₁. The predictions of catalytic triads (serine, histidine and asparagine) were also visualized in the peak level obtained in infra-red (IR) and H₁ nuclear magnetic resonance (NMR) spectra. With this information it was suggested that, partial characterization of catalytic domain was predicted in the fungal isolates Ifr.