African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Assessment of genetic diversity among accessions of two traditional leafy vegetables (Acmella uliginosa (L.) and Justicia tenella (Nees) T.) consumed in Benin using amplified fragment length polymorphism (AFLP) markers

Adéoti K.1, Rival A.2, Dansi A.3*, Ahohuendo B.C4, Santoni S.5, Beule T.2, Nato A.6, Henry Y.7, Ahanchédé A.4, Vodouhè R.8, Hounhouigan D.J.4 and Sanni A.1
1Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi (UAC), BP 526 Cotonou, Republic of Benin. 2Cirad-BioS, UMR DIAPC, Centre IRD. 34394 Montpellier Cedex 15, France. 3Laboratory of Agricultural Biodiversity and Tropical Plant Breeding, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi (UAC), 071BP28, Cotonou, Republic of Benin. 4Faculty of Agriculture (FSA), University of Abomey-Calavi (UAC), BP 526 Cotonou, Republic of Benin. 5Centre INRA, UMR DIAPC, Unité de Génétique et Amélioration des plantes, Atelier de Marquage Moléculaire, 34060 Montpellier Cedex 01, France. 6Institut de Génétique et Microbiologie, Bâtiment 360, Université Paris-Sud 11, 91405, Orsay, France. 7Institut de Biotechnologie des Plantes, Bâtiment 630, Université Paris-Sud 11, 91405 Orsay, France. 8Bioversity International, Office of West and Central Africa, 08 BP 0931, Cotonou, Republic of Benin.
Email: [email protected]; [email protected]

  •  Accepted: 18 February 2011
  •  Published: 11 April 2011

Abstract

Amplified fragment length polymorphism (AFLP) markers were used to evaluate the genetic diversity and explore the genetic relationship among accessions of Acmella uliginosa and Justicia tenella, two leaf vegetables collected from different areas in the northwest and northeast parts of Benin (West Africa). The total number of exploitable amplicons revealed with genomic DNA from A. uliginosa was 224 with an average of 50.5% polymorphic amplicons. Using DNA from J. tenella, we obtained 34% of polymorphic amplicons from a total of 418. The coefficient of dissimilarity varied from 0.01 to 0.67 and from 0.17 to 0.62 for Acmella and Justicia, respectively. Low genetic diversity was observed among Acmella accessions although three distinct clusters could be differentiated. Contrarily, a great genetic diversity was observed among J. tenella accessions. In addition to this, most of the clusters were heterogeneous and showed the relationship between accessions collected from northeast and northwest. Our results confirm the robustness of AFLP techniques for genetic diversity studies and they provide the first set of molecular data for these two species.

 

Key words: Amplified fragment length polymorphism (AFLP), genetic diversity, leafy vegetable, Benin.

Abbreviation

AFLP, Amplified fragment length polymorphism; TLVs, traditional leafy vegetables; NUS, underutilized species.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 10 No. 15
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES