African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Antibacterial action of an aqueous grape seed polyphenolic extract

  Veronica Sanda Chedea1,5*, Cornelia Braicu2, Flore ChirilÇŽ3, Ciprian Ober4 and Carmen Socaciu1
  1Department of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania. 2Cancer  Institute “I. Chiricuta” Cluj-Napoca, 34-36 Republicii Str., Cluj-Napoca, Romania. 3Depatment of Microbiology-Immunology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania 4Department of Anesthesiology and Surgical Propedeutics, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur Str., 400372 Cluj-Napoca, Romania. 5Laboratory of Animal Biology, National Research Development Institute  for Animal Biology and Nutrition (IBNA), Calea Bucuresti 1, 077015 Balotesti, Romania.
Email: [email protected]

  •  Accepted: 25 March 2011
  •  Published: 06 July 2011

Abstract

 

The potential of a polyphenolic grape seed extract for use as a natural antibacterial agent was evaluated. Pure catechin (CS) and a previously LC-MS characterized grape seed phenolic extract (PE) were evaluated as antibacterial agents againstEscherichia coli and Brevibacterium linens on solid and in liquid culture media. After 48 h incubation on solid medium, PE had a bactericidal effect on the gram positiveB. linens and a reduction of the microbial growth for E. coli. The antibacterial agents tested were effective against E. coli for 13 h, after 7 h incubation, but ineffective against B.linens in liquid medium. CS and PE both had an antibacterial effect depending on incubation time.

 

Key words: Catechin, polyphenolic extract, antibacterial activity, Brevibacterium linens, Escherichia coli B41.

Abbreviation

CS, Catechin standard; UV-Vis, spectroscopy ultraviolet-visible spectroscopy; PE, grape seed polyphenolic extract; LC-UV-DAD, liquid chromatography with ultraviolet diode array detection; LC-ESI-MS, liquid chromatography-electrospray ionisation-mass spectrometry; PBS, phosphate buffered saline; KOH, potassium hydroxide.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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