Full Length Research Paper
Abstract
The potential of a polyphenolic grape seed extract for use as a natural antibacterial agent was evaluated. Pure catechin (CS) and a previously LC-MS characterized grape seed phenolic extract (PE) were evaluated as antibacterial agents againstEscherichia coli and Brevibacterium linens on solid and in liquid culture media. After 48 h incubation on solid medium, PE had a bactericidal effect on the gram positiveB. linens and a reduction of the microbial growth for E. coli. The antibacterial agents tested were effective against E. coli for 13 h, after 7 h incubation, but ineffective against B.linens in liquid medium. CS and PE both had an antibacterial effect depending on incubation time.
Key words: Catechin, polyphenolic extract, antibacterial activity, Brevibacterium linens, Escherichia coli B41.
Abbreviation
CS, Catechin standard; UV-Vis, spectroscopy ultraviolet-visible spectroscopy; PE, grape seed polyphenolic extract; LC-UV-DAD, liquid chromatography with ultraviolet diode array detection; LC-ESI-MS, liquid chromatography-electrospray ionisation-mass spectrometry; PBS, phosphate buffered saline; KOH, potassium hydroxide.
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