African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Cloning and characterization of a cDNA encoding phytoene synthase (PSY) in tea

D. Borthakur, Y. Y. Du, H. Chen, J. L. Lu, C. Lin, J. J. Dong, J. H. Ye, X. Q. Zheng and Y. R. Liang*
Zhejiang University Tea Research Institute, 268 Kaixuan Road, Hangzhou 310029, China.
Email: [email protected]

  •  Accepted: 09 September 2008
  •  Published: 20 October 2008

Abstract

Carotenoids are an important group of precursors of volatile flavour compounds in tea and phytoene synthase (PSY) is a key enzyme during the biosynthesis pathway of carotenoids. A cDNA encoding PSY in tea shoot [Camellia sinensis (L.) O. Kuntze] was cloned and sequenced in the present study. The obtained tea PSY cDNA was 1296 bp with an open reading frame of 987 bp. The predicted protein displayed a sequence of 329 amino acids. The phylogenetic tree showed that thetea PSY was clustered closely to the PSY of Adonis palaestina, with an overall amino acid identity of 90%.

 

Key words: Camellia sinensis, carotenoids, phytoene synthase, gene cloning, sequencing, phylogenetic tree.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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