African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Antioxidant properties of banana flower of two cultivars in China using 2,2-diphenyl-1-picrylhydrazyl (DPPH,) reducing power, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) and inhibition of lipid peroxidation assays

Zhan-Wu Sheng1, Wei-Hong Ma1, Jin-He Gao1, Yang Bi2, Wei-Min Zhang3, Hua-Ting Dou4, Zhi-Qiang Jin1*
1Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, 570102 Haikou, Hainan, P. R. China. 2College of Food Science and Engineering, Gansu Agriculture University, 730070 Lanzhou, Gansu, P. R. China. 3College of Food Science, Hainan University, 570028 Haikou, Hainan, P. R. China. 4Department of Citrus, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Email: [email protected]

  •  Accepted: 31 January 2011
  •  Published: 23 May 2011

Abstract

In this study, the antioxidant properties of banana flower extracts (cvs. Baxijiao (AAA) and Paradisiaca   (AAB)) were analysed by using several biochemical assays which include 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, 2, 2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) radical scavenging activities and inhibition of lipid peroxidation in egg lecithin through the formation of thiobarbituric acid-reactive substances (TBARS). These assays have been extensively studied and generally accepted as models to characterize peroxidative damage in biomembranes. In the present study, the EC50 values were calculated using each method as listed above was used to compare the antioxidant efficiency of each banana flower extract. The phenol, flavonoid, vitamin E and saponin contents were also analyzed. Baxijiao flower extract revealed better antioxidant properties by presenting much lower EC50 values, particularly for reducing power. In addition, antioxidant concentrations (polyphenols and flavonoids) were found higher in this flower sample than those in the Paradisiaca sample. The results suggested that the Baxijiao flower could be a better resource either as a dietary supplement or as a food additive than the later one.

 

Key words: Banana flower, antioxidant, scavenging effects, peroxidation.

Abbreviation

Abbreviations: ABTS, 2,2’-Azinobis-(3-ethylbenzthiazoline-6-sulphonate; TBARS,thiobarbituric acid-reactive substances; ROS, reactive oxygen species; DPPH, 1,1-diphenyl-2-picrylhydrazyl; HPLC, high performance liquid chromatography; CEQ,catechin equivalents; RSA, radical-scavenging activity; GAE, gallic acid equivalents.