African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Novel approach for optimization of fermentative condition for polyhydroxybutyrate (PHB) production by Alcaligenes sp. using Taguchi (DOE) methodology

Abhishek Dutt Tripathi* and S. K. Srivastava
School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
Email: [email protected]

  •  Accepted: 03 June 2011
  •  Published: 20 July 2011

Abstract

Polyhydroxybutyrate (PHB) is a biodegradable thermoplastic polymer which is accumulated as energy reserve material by large number of microorganisms including bacteria, fungus and yeast under nutrient stress condition. In this study, efforts have been made to optimize PHB production by Alcaligenes sp. NCIM 5085 using Taguchi (DOE) methodology. This approach facilitates the study of interaction of a large number of variables spanned by factors and their settings with a smaller number of experiments, leading to considerable savings in time and cost for process optimization. Eight factors at two levels with an OA layout of L-12 were selected for proposed experimental design. PHB concentration was increased from 5.20 to 6.58 g/L under optimal cultural condition. Result validation showed 95% resemblance with the expected value.

 

Key words: Polyhydroxybutyrate, Alcaligenes sp. Taguchi (DOE), orthogonal arrays layout.

Abbreviation

OA, Orthogonal arrays; DOE, design of experiment; PHB, polyhydroxybutyrate.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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