Potentiality of benthic dinoflagellate cultures and screening of their bioactivities in Jeju Island, Korea

Md. Mahfuzur Rahman Shah; Kalpa W. Samarakoon; Ju-Young Ko; H. H. Chaminda Lakmal; Ji-Hyeok Lee; So-Jeong An; You-Jin Jeon; Joon-Baek Lee

doi:10.5897/AJB2013.13250

African Journal of
Biotechnology

Abbreviation: Afr. J. Biotechnol.
Language: English
ISSN: 1684-5315
DOI: 10.5897/AJB
Start Year: 2002
Published Articles: 12487

Full Length Research Paper

Potentiality of benthic dinoflagellate cultures and screening of their bioactivities in Jeju Island, Korea

Md. Mahfuzur Rahman Shah

Md. Mahfuzur Rahman Shah
Jeju Sea Grant Center, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea
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Kalpa W. Samarakoon

Kalpa W. Samarakoon
Department of Marine Life Science, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea
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Ju-Young Ko

Ju-Young Ko
Department of Marine Life Science, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea
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H. H. Chaminda Lakmal

H. H. Chaminda Lakmal
Department of Marine Life Science, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea
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Ji-Hyeok Lee

Ji-Hyeok Lee
Department of Marine Life Science, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea
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So-Jeong An

So-Jeong An
Department of Earth and Marine Sciences, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea.
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You-Jin Jeon

You-Jin Jeon
Department of Marine Life Science, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea
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Joon-Baek Lee

Joon-Baek Lee
Department of Earth and Marine Sciences, College of Ocean Sciences, Jeju National University, Jeju 690-756, Korea.
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Article Number - 2AB02FA42988
Vol.13(6), pp. 792-805 , February 2014
https://doi.org/10.5897/AJB2013.13250

Received: 10 August 2013
Accepted: 24 January 2014
Published: 05 February 2014

Abstract

Eleven strains of benthic dinoflagellates (Amphidinium carterae (D1), Prorocentrum rhathymum (D2), Symbiodinium sp. (D3), Coolia malayensis 1 (D4), Ostreopsis ovata 1 (D5), Ostreopsis ovata 2 (D6), Coolia malayensis 2 (D7), Amphidinium operculatum 1 (D8), Heterocapsa psammophila (D9), Coolia malayensis 3 (D10) and Amphidinium operculatum 2 (D11)) were collected in Jeju Island, Korea and cultured in 20 L carboys after establishing unialgal cultures. Their growth potential and biomass productivity were evaluated using two different culture media (IMK and f/2 medium); it was found that IMK medium has the potential to culture benthic dinoflagellates compared to commonly used f/2 medium. Among the benthic dinoflagellates, A. carterae (D1) had the maximum cell density (148.6 × 10³ cells mL^-1), growth rate (0.317 ± 0.01 divisions day^-1) and biomass (0.260 ± 0.03 g L^-1 dry weight) in IMK medium at 20 days of culture. Also, screened bioactivities among the methanolic extracts of cultured dinoflagellates showed A. carterae (D1) to have the highest antioxidant and anti-inflammatory effect and O. ovata 1 (D5) had the highest anticancer activity compared to the other strains. Taken together, this is the first report on the growth potential and biomass production of benthic dinoflagellate strains isolated from Jeju Island in appropriate culture medium as well as their importance in potential pharmacological applications.

Key words: Amphidinium carterae, benthic dinoflagellates, biomass, bioactivities, culture conditions, Jeju Island.

Abbreviation

Abbreviations: PSP, paralytic shellfish poisoning; NSP, neurotoxic shellfish poisoning; AZP, azaspiracid poisoning; DSP, diarrheic shellfish poisoning; CFP, ciguatera fish poisoning; DPPH, 1,1-diphenyl-2-pricrylhydrazyl; ESR, electro spin resonance; DMEM, Dulbecco's modified eagle medium; RPMI, Roswell park memorial institute; FBS, fetal bovine serum; NO, nitric oxide; ELISA, enzyme-linked immunosorbent assay; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide; DMSO, dimethylsulfoxide.