African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Analysis of mtDNA hypervariable region II for increasing the discrimination power from Middle and South of Iraq

Ameera Omran Hussein
  • Ameera Omran Hussein
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Muhanned Abdulhasan Kareem
  • Muhanned Abdulhasan Kareem
  • Babylon University, Centre of Environmental Research, Iraq.
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Imad Hadi Hameed*
  • Imad Hadi Hameed*
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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  •  Received: 08 February 2015
  •  Accepted: 02 March 2015
  •  Published: 11 March 2015

Abstract

Mitochondrial DNA is a useful genetic marker for answering evolutionary questions due to its high copy number, maternal mode of inheritance, and its high rate of evolution. The aims of this research were to study the mitochondria noncoding region by using the sanger sequencing technique and establish the degree of variation characteristic of a fragment FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. A portion of a non-coding region encompassing positions 37 to 340 for HVII was amplified in accordance with the Anderson reference sequence. PCR products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3730xL DNA analyzer. New polymorphic positions G92C, C113G, C150G, T156A, C194G, C198G, G207C, G225C and G228C are described and may in future be suitable sources for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence most promising for identification variants.

 

Key words:  D-loop, HVII, Middle and South of Iraq, mitochondrial DNA. 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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