Abstract

Propagation methods do not exist for Securidaca longepedunculata. In this study,ex vitro and in vitro experiments were conducted to identify simple propagation methods for this species. Ex vitro germination rates did not exceed 43%, whereasin vitro procedures achieved 67 to 90% germination rates. In vitro germinated seedlings produced at least two re-sprouts and consistently showed vigorous growth. Explants cultured on B5 medium supplemented with indole-3-butyric acid (IBA) and 1-naphthalene-3-acetic acid (NAA) successfully produced roots within 4 weeks. It is concluded that germination of seeds, shoot multiplication and rooting ofS. longepedunculata can be improved through appropriate in vitro procedures.

Key words: Agroforestry, axillary shoot multiplication, ex vitro germination, gibberellic acid, in vitro propagation.

Abbreviation

B5, Gamborg’s B5 medium; GA, gibberelic acid; IBA, indole-3-butyric acid; 2ip, isopentenyladenine; MS, Murashige and Skoog’s medium; NAA, 1-naphthalene-3-acetic acid; SDICN, sodium dichloroisocyanurate; TDZ, thidiazuron.