African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Antimicrobial activities of different solvents extracted samples of Linum usitatissimum by disc diffusion method

Jehan Bakht1*, Huma Ali1, Mubarak Ali Khan2, Ayub Khan3, Muhammad Saeed3, Mohammad Shafi4, Amjad Islam1 and Mohammad Tayyab1
1Institute of Biotechnology and Genetic Engineering KPK Agricultural University Peshawar Pakistan. 2Department of Biotechnology, Qauid-E-Azam University Islamabad Pakistan. 3Department of Agriculture, Hazara University Haripur Campus KPK Pakistan. 4Department of Agronomy, KPK Agricultural University Peshawar Pakistan.
Email: [email protected]

  •  Accepted: 07 September 2011
  •  Published: 28 December 2011

Abstract

This research work was carried out to investigate the antimicrobial activities of different solvents extracted samples of Linum usitatissimum against seven bacterial and one fungal pathogen. Three concentrations (0.66, 1.00 and 1.33 mg dics-1) each of ethyl acetate, n-hexane, butanol and distilled water were used. Analysis of the data revealed that the growth of Staphylococcus aureus was inhibited equally by butanol and ethyl acetate extracted samples that is, 56% when tested by disc diffusion method. Similarly, ethyl acetate and butanol extracted samples reduced the growth of Bacillus cereus by 44 and 64% respectively. The data further suggest that Erwinia carotovora was inhibited 42% by ethyl acetate and 65% by butanol extracted samples.

 

Key words: Antimicrobial, solvent, Linum, disc diffusion.