African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular cloning and functional analysis of the follicle-stimulating hormone (FSH) receptor gene promoter from the Jintang black goat

Cao Ye1,2, Zhao Sujun1,2, Liang Tianyu3, Qian Shaoping4 and Zhang Yizheng1*
  1College of Life Science, Sichuan University, Chengdu, China 610064. 2Sichuan Academy of Animal Science, Chengdu, China 610061. 3Southwest University for Nationalities, Chengdu, China 610041. 4Chengdu Railway Central Hospital, Chengdu, China 610081.
Email: [email protected]

  •  Accepted: 06 May 2011
  •  Published: 28 September 2011

Abstract

 

A 762 bp fragment of the 5’-flanking region of the FSHR gene from the Jintang black goat was cloned. The putative initial transcript site was the A at 681 bp and there were 7 putative cis-acting elements and 3 AT-rich regions. The sequence of theFSHR promoter from the Jintang black goat is 99.34% homology to Capra hircus, 32.38% to Gallus gallus and 38.55% to mouse. It could promote the EGFP, FSHR transcription in HEK293 cells, the fluorescence intensity was weaker than the CMV promoter, but the expressed FSHR could respond to the FSH signaling with signal intensity much higher than that at 24 h. This indicated that the FSHR promoter of the Jintang black goat is a strong promoter and may be a gene-special promoter.

 

Key words: Follicle-stimulating hormone receptor, gene promoter, Jintang black goat, molecular cloning, functional analysis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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