African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Transformation of trehalose synthase gene (TPS Gene) into corn inbred line and identification of drought tolerance

Dong Chun-lin1, Zhang Ming-yi1, Zhang Yan-qin1, Yang Li-li1, Liang Gai-mei1, Sun Jie1, Lin Zhong-ping2 and Gou Jjian-fang3
1Dryland Agriculture Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China. 2College of Life Sciences, Peking University, Beijing 100871, China. 3Information Institute Shanxi Academy of Agricultural Science, Taiyuan 030006, China.
Email: [email protected]

  •  Accepted: 26 September 2011
  •  Published: 30 November 2012

Abstract

Trehalose synthase gene (TPS gene) was transferred into maize inbred line Zheng58 with the improved pollen tube path method. At the three to five leaf stage, selection with spray Basta (0.7%) solution was conducted, and was then followed by polymerase chain reaction (PCR) and PCR-southern detection. The results showed that the new gene was transferred into the plants and integrated into the plant chromosomes. The initial drought-tolerance test in farmland confirmed that the drought resistance of transgenic plants was higher than that of non-transgenic plants. The results show that the new maize drought resistance germplasm can be gained by means of pollen tube path which is a feasible, effective and new way for maize drought resistance breeding.

 

Key words: Maize, trehalose synthase gene (TPS Gene), drought resistance.

Abbreviation

TPS, Trehalose synthase; PCR, polymerase chain reaction; PPT,phosphinothricin.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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