African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

First report of mortality in Phoenix canariensis associated with subgroup 16SrIV‐D phytoplasmas in Coahuila, Mexico

Pablo José Palma-Cancino
  • Pablo José Palma-Cancino
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán A. C., Mérida, Yucatán, México.
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José Alfredo Samaniego-Gaxiola
  • José Alfredo Samaniego-Gaxiola
  • Campo Experimental La Laguna, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Matamoros, Coahuila, México.
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María Narváez
  • María Narváez
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán A. C., Mérida, Yucatán, México.
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Germán Nic-Matos
  • Germán Nic-Matos
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán A. C., Mérida, Yucatán, México.
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Yasmín Chew-Madinaveitia
  • Yasmín Chew-Madinaveitia
  • Campo Experimental La Laguna, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Matamoros, Coahuila, México.
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Aurelio Pedroza-Sandoval
  • Aurelio Pedroza-Sandoval
  • Unidad Regional Universitaria de Zonas Áridas, Universidad Autónoma Chapingo, Bermejillo, Durango, México.
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Arturo Gaytán-Mascorro
  • Arturo Gaytán-Mascorro
  • Departamento de Fitomejoramiento, Unidad Laguna, Universidad Autónoma Agraria Antonio Narro, Torreón, Coahuila, México.
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Carlos Oropeza
  • Carlos Oropeza
  • Unidad de Biotecnología, Centro de Investigación Científica de Yucatán A. C., Mérida, Yucatán, México.
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  •  Received: 23 June 2020
  •  Accepted: 20 August 2020
  •  Published: 31 December 2020

Abstract

In 2015, a disease of unknown origin appeared in Torreon, Coahuila, in the Northeast of Mexico, causing great mortality in Phoenix palms, especially in Phoenix canariensis. Until early 2019, around 1300 palms died from this disease. The aim of this study was to determine its etiology. The symptoms registered in affected palms were similar to those described for Texas Phoenix Palm Decline (TPPD). Phytoplasmas were detected in samples from nine P. canariensis individuals using a TaqMan/real-time PCR assay specific for group 16SrIV detection. DNA of positive samples was amplified by nested PCR using primer pair P1/P7 followed by LY16Sf/LY16-23Sr and R16F2n/R16R2. In silico analysis of the sequences obtained revealed the presence of phytoplasmas associated with TPPD, belonging to subgroup 16SrIV-D. This is the first report of a disease associated with subgroup 16SrIV-D phytoplasmas in the Northeast of Mexico, further extending the known geographical range of this pathogen.

 

Key words: Texas Phoenix palm decline, 16SrIV-D phytoplasmas, nested polymerase chain reaction (PCR), sequence analysis.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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