Abstract

Dried ground leaves and rhizomes of Typha capensis were extracted with different solvents of varying polarity (hexane, dichloromethane, acetone, methanol) to determine the best extractant for subsequent isolation and characterization of antibacterial compounds. Some extracts were active against Escherichia coli andEnterococcus faecalis, with at least one of them exhibiting minimum inhibitory concentration values of 0.04 mg/ml. Methanol was the best extractant with an average minimum inhibitory concentration (MIC) value of 0.75 mg/ml (rhizome) and 0.21 mg/ml (leaves) for the four pathogens. E. coli and E. faecalis were the most sensitive with the average MIC values of 0.53 and 0.42 mg/ml, respectively.Staphylococcus aureus was the most resistant pathogen. The MIC values for the positive control (ampicillin) were ranging from 0.08 to 0.16 mg/ml. Average total activity, a measure of potency, was highest for methanol (4498 ml/g) leaves and (1838 ml/g) rhizomes extract followed by acetone (1795 ml/g) leaves and (1075 ml/g) rhizome extracts. The most active compounds were at the R_f value of 0.47 against all pathogens. In some extracts the antibacterial activity was high enough to consider extracts for isolation and characterisation of antibacterial compounds.

Key words: Typha capensis, typhaceae, antibacterial, bioautography.