African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Enzyme immunoassay measurements of the molting hormone in different post-embryonic stages of two mosquito species, Culex pipiens and Culiseta longiareolata

Fouzia Tine-Djebbar, Aicha Beya Larhem and Noureddine Soltani*
Laboratoire de Biologie Animale Appliquée, Département de Biologie, Faculté des Sciences, Université Badji Mokhtar, 23000-Annaba, Algérie.
Email: [email protected]

  •  Accepted: 15 September 2011
  •  Published: 31 October 2011

Abstract

Due to their large geographical distribution, abundance and harmfulness, Culex pipiens L. and Culiseta longiareolata Macquart are the two most remarkablemosquito species in Algeria. The nature and the level of ecdysteroids from whole body extracts of different post-embryonic stages of the two mosquito species was made by an enzyme-immunoassay (EIA) using two specific antibodies, the rat monoclonal EC 19 antibody showing a high affinity for 20-hydroxyecdysone (20E) and the rabbit polyclonal B antibody with a strong affinity for ecdysone (E). EIA measurements confirmed the presence of two main hormones in the two mosquito species: E and 20E. Moreover, there is a predominance of 20E.

 

Key words: Mosquitoes, Culex pipiens, Culiseta longiareolata, hormone, enzyme-immunoassay (EIA).

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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