African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Phytochemical analysis of selected medicinal plants

Iqbal Hussain*1, Riaz Ullah1,6, Rooh Ullah2 , Muhammad Khurram3, Naseem Ullah3, Abdul Baseer3, Farhat Ali Khan3, Muneeb ur Rehman Khattak1, Mohammad Zahoor4, Jehangir Khan5 and Naeem khan1
1Department of Chemistry, Kohat University of Science & Technology, KPK, Pakistan. 2Institute of Chemical Sciences, University of Peshawar, KPK, Pakistan. 3Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, KPK, Pakistan. 4Department of Chemistry, University of Malakand, Malakand, KPK, Pakistan. 5Department of Pharmacy, University of Malakand, Malakand, KPK, Pakistan. 6Department of Chemical Engineering, College of Engineering, King Saud University, P.O BOX 800, Riyadh 11421, Saudi Aarbia
Email: [email protected]

  •  Accepted: 06 April 2011
  •  Published: 25 July 2011

Abstract

Four medicinal plants including Ranunculus arvensisEquisetum ravens,Carathamus lanatus and Fagonia critica were used for the study. All the plants were biologically active and were used for different types of ailments. Keeping in view their importance, this work was carried out to investigate the quantitative determination of their crude phytochemicals, vitamins and protein contents. The quantitative determination of crude phytochemicals (alkaloids, total phenols, flavonoids and saponins) vitamins (riboflavin, vitamin C, niacin and pectin) and protein were determined in the aforementioned herbs. The phytochemicals including alkaloids, total phenols, flavonoids and saponins were determined quantitatively using literature methods. Vitamins were measured using a UV/ visible spectrophotometer (UV- 1601 Shamidzu) and the protein was determined by the Micro KJeldahl’s method (Horwitz et al., 2000). The studied plants showed variable amounts of phytochemicals, vitamins and protein contents. The study is very important, in that it intended to show the contents of the studied medicinal herbs and also provide a scientific data base line which is of particular importance for the local practioners as well as for the local people using these herbs for a variety of body disorders.

 

Key word:  Phytochemical analysis, medicinal plants, Pakistan.