African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

High level production of the recombinant gag24 protein from HIV-1 IN Escherichia coli

Ramón E. Narciandi Díaz
  • Ramón E. Narciandi Díaz
  • Department of Technology Transfer, Center for Genetic Engineering and Biotechnology, Ave 31 e/158-190, Playa, P.O.Box 6162, Havana 10600, Cuba.
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José Motolongo Viquillon
  • José Motolongo Viquillon
  • Division of Vaccine HBsAg, Center for Genetic Engineering and Biotechnology, Ave 31 e/158-190, Playa, P.O.Box 6162, Havana 10600, Cuba
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Luis Pérez Figueroa
  • Luis Pérez Figueroa
  • Division of Vaccine HBsAg, Center for Genetic Engineering and Biotechnology, Ave 31 e/158-190, Playa, P.O.Box 6162, Havana 10600, Cuba
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  •  Accepted: 28 July 2005
  •  Published: 16 January 2006

Abstract

The gag24 gene of the Human Immunodeficiency Virus type 1 (HIV-1) was expressed under the control of the tryptophan promoter in Escherichia coli. The effect of several parameters on the production of gag24 was studied. The expression level achieved (25%) depended on the host strain and the induction conditions. The developed fermentative process was scaled-up to 50 L, where 417 mg/L of volumetric production and 34 mg/L.h of productivity, were obtained.

 

Key words: Escherichia coli, gag24, HIV-1, scale-up.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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