African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Short Communication

Screening and characterization a RAPD marker of tobacco brown-spot resistant gene

H. Y. Zhang1, Y. M. Yang1, F. S. Li2, C. S. He3 and X. Z. Liu1*
1Biotechnology Laboratory, Southwest Forestry College, Kunming, 650224 Yunnan Province, People’s Republic of China. 2College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201Yunnan Province, People’s Republic of China. 3The South Center of Tobacco Breeding Research of China, Yuxi, 653100 Yunnan, People’s Republic of China.
Email: [email protected]

  •  Accepted: 02 July 2008
  •  Published: 04 August 2008

Abstract

Bulk Segregant Analysis (BSA) and Randomly Amplified Polymorphic DNA (RAPD) methods were used to analyze F2 individuals of 82-3041 × Yunyan 84 to screen and characterize the molecular marker linked to brown-spot resistant gene. A total of 800 arbitrary decamer oligonucleotide primers were used for RAPD analysis. Primer S361, producing one RAPD marker S361650, was tightly linked to the brown-spot resistant gene. Linkage analysis was carried out using marker S361650 on 1042 individuals of F2 progenies from the crossing between 82-3041 × Yunyan 84. The results demonstrated that the genetic distances between S361650and brown-spot resistant gene was 2.98 cM.

 

Key words: Brown-spot, resistance gene, bulk segregant analysis, molecular marker, and linkage analysis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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