African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Studies on improved agrobacterium - mediated transformation in two indica rice (Oryza Sativa L.)

Vinod Saharan1, Ram C. Yadav2, Neelam R. Yadav2 and Khushi Ram3
1The Institutes for Applied Research, Ben-Gurion University, P.O.B. 653, Beer-Sheva 84105, Israel. 2Department of Biotechnology and Molecular Biology, CCS HAU, Hisar 125004 (Haryana), India. 3Rice Research Station, Kaul, (Haryana), India.
Email: [email protected]

  •  Accepted: 09 September 2004
  •  Published: 30 November 2004

Abstract

Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1301 was used for transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR-126. High concentrations of acetosyringone in theagrobacterium culture and co-cultivation medium proved to be indispensable for successful transformation. Embryogenic scutellar calli were used for transformation studies. Binary vector pCAMBIA 1301 have been proved efficient for transformation. The percent transient GUS expression found to be higher in cutivar HKR-126 (44.4%) as compared to HKR-46 (28.9%). The percent recovery of hygromycin resistant calli after 4-6 weeks on selection medium was maximum in HKR-126 (52.6%).

 

Key words: Agrobacterium, indica rice, transformation, acetosyringone.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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