Abstract

Leaf explants and mature embryos of Datura were separately cultured in  MS basal medium supplemented with 30 g/l sucrose, 8 g/l agar and different combination of 2,4,D (at three levels, 0, 1 and 2 mg/l) and kinetin (0, 0.25, 0.5 and 1 mg/l). The formed calli were transferred to regeneration media supplemented with BAP alone (at three levels, 1, 2 and 3 mg/l) or in combination with NAA (at four levels, 0, 0.02, 0.2 and 1 mg/l). The regenerated shoots were rooted in media containing IBA at three levels (0.5, 1 and 1.5 mg/l). The media containing 2 mg/l 2,4-D + 0.5 mg/l kinetin and 2 mg/l of 2,4-D alone were found to be the best treatments for callus induction  from leaf and embryo explants, respectively.  Moreover, the media containing 3 mg/l BAP + 1 mg/l NAA and 2 mg/l BAP + 1 mg/l NAA were found to be the best hormonal treatments to shoot regeneration from calli of leaf and embryo explants, respectively.  Also 0.5 mg/l IBA was found to be the best treatment for rooting of regenerated shoots.

Key words: Datura, leaf explants, embryo explants, calli.

Abbreviation

Abbreviations: 2,4-D, 2,4-Dichloro-phenoxyacetic acid; MS, murashige and skoog medium; BAP, 6-benzylaminopurine; IBA, indole-3-butyric acid; NAA, α- naphthalene acetic acid.