African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Effects of zerumbone on cisplatin-induced clastogenesis in Sprague-Dawley rats bone marrow cells

Adel S. Al-Zubairi1,3*, Ahmad B. Abdul1,2 and Mohammed Yousif1    
1Laboratory of Cancer Research, MAKNA-UPM, Institute of Biosciences (IBS), University Putra Malaysia, Serdang, 43400, Selangor D.E., Malaysia. 2Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, 43400, Selangor D.E., Malaysia. 3Department of Biochemistry and Molecular Biology, Faculty of Medicine and Health Sciences, University of Sana’a, Sana’a, Yemen.    
Email: [email protected]

  •  Accepted: 18 November 2010
  •  Published: 20 June 2011

Abstract

Zerumbone (ZER) is derived from Zingiber zerumbet smith from the Zingiberaceae family. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour cells. The aim of our study was to assess the effect of ZER on cisplatin-induced clastogenesis in Sprague-Dawley rat bone marrow polychromatic erythrocytes (PCEs) using micronucleus test (MN). Animals treated with two ZER doses for 4 consecutive days plus a single dose of cisplatin following treatment, presented a non-significant effects of ZER on cisplatin-induced clastogenesis. The results also indicated that ZER has no clastogenic effects on rat bone marrow polychromatic erythrocytes after 4 days treatment with 250 and 500 mg/kg body weight when compared to one dose cisplatin of 45 mg/kg body weight.On the other hand, significant decrease in the number of PCE was observed in alltreatment groups, indicating cytotoxicity of ZER and cisplatin. Under the present experimental conditions, ZER could not prevent cisplatin-induced clastogenesis in rat.

 

Key words: Clastogenicity, micronucleus, micronuclei in polychromatic erythrocytes (MNPCEs), zerumbone.

Abbreviation

MNPCEs, Micronuclei in polychromatic erythrocytes; ZER,zerumbone; CC, column chromatography; HPLC, high performance liquid chromatography; LCMS, liquid chromatography mass spectrometry; MTD, maximum tolerable dose; DMSO, dimethyl sulfoxide; NCE, normochromatic erythrocytes; PCE,polychromatic erythrocytes; MN, micronuclei.