African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Evaluation of the genetic structure of the urban dwelling species of Bank Myna (Acridotheres ginginisnus) using random amplified polymorphic DNA (RAPD) analysis

  Noreen Akhter1, Aleem Ahmed Khan1, Muhammad Babar2, Maria Riaz1, Ayesha Imtiaz1 and Imran Khaliq1,3*
  1Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan. 2Institute of Biotechnology, Bahauddin Zakariya University, Multan, Pakistan. 3Biology Department, Government Post Graduate College, Dera Ghazi Khan, Pakistan.
Email: [email protected]

  •  Accepted: 13 May 2011
  •  Published: 06 July 2011

Abstract

 

We used the random amplified polymorphic DNA (RAPD) technique to decipher the genetic structure of the Bank Myna (Acridotheres ginginisnus) in Pakistan. The samples were collected from four cities namely: Dera Ghazi Khan, Jahanian, Khanewal and Gujranwala. The analysis showed a high genetic diversity at species level (H = 0.318, S = 0.467) but low levels at population levels (H = 0.047, S = 0.069 to H = 0.195, S = 0.283). The four populations analyzed were genetically distant from each other and hinted the effect of urbanization role in isolating the urban dwelling species. The genetic distances between populations ranged from D = 0.2403 to D = 0.3419 and the similarity coefficient showed low range from 0.66 to 0.70. This study will help in future conservation plans and also help the understanding of the role of urbanization on fragmentation of species’ natural distribution and dispersal mechanism.

 

Key words: Acridotheres ginginisnus, Bank Myna, random amplified polymorphic DNA (RAPD), genetic structure.