African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Preliminary research of recombinant matrix extracellular phosphoglycoprotein (MEPE) mineralization ability in vitro

  Ai Lin1, Wang Huiyuan2*, Hu Hua3, Liu Yanqing4 , Ma Tiehua1 and Zhai Jingyun1
  1Department of Stomatology, People’s Liberation Army No.12 Hosipital. Kuonabazha Road 13. Kashi, 844200, China. 2Department of Stomatology, People’s Liberation Army No.323 Hosipital. Jianshexi Road 66. Xi’an, 710048, China. 3Department of institution, People’s Liberation Army No.12 Hosipital. Kuonabazha Road 13. Kashi, 844200, China. 4Out-patient Department, People’s Liberation Army No.12 Hosipital. Kuonabazha Road 13. Kashi, 844200, China.
Email: [email protected]

  •  Accepted: 13 April 2011
  •  Published: 11 July 2011

Abstract

 

The matrix extracellular phosphoglycoprotein (MEPE) gene is highly expressed in tumors that cause oncogenic hypophosphatemic osteomalacia (OHO). MEPE is also known as one of the bone-tooth matrix proteins and is associated with bone and teeth mineralization. We developed a rabbit polyclonal antibody directed against recombinant human MEPE after cloning its cDNA from the cDNA library of a human brain cDNA library. Using this anti-body, we analyzed the distribution of MEPE in dog dental germ tissue by immunohistochemistry. In these specimens, MEPE was predominantly expressed by odontoblast cells and predentin, but not by dental pulp cells. Furthermore, we used von kossa staining and the results suggested that, MEPE could induce mineralization and we propose that this protein had a potential effect on dental rehabilitation.

 

Key words: Matrix extracellular phosphoglycoprotein (MEPE), mineralization Von kossa.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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