African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

High-frequency shoot regeneration of nodal explants from Tetrastigma hemsleyanum Diels et Gilg: A valuable medicinal plant

Weimei Jiang1,2, Yuanmiao Fu1,2, Xiaolong Zhou3 and Chengxin Fu1,2*
1The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, China. 2Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Plant Sciences and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, China. 3Pan’an Institute of Traditional Chinese Medicine, Pan’an 322300, China.
Email: [email protected]

  •  Accepted: 29 June 2011
  •  Published: 28 September 2011

Abstract

 This paper describes the shoot regeneration of nodal segments from a medicinalplant, Tetrastigma hemsleyanum Diels et Gilg (Vitaceae). The highest number of shoots (7.27 shoots per explant) was observed in MS medium supplemented with 4 mg/l BA after six weeks of inoculation. 2 mg/l BA in combination with 0.1 mg/l NAA not only induced shoot proliferation but also increased shoot length. Well-developed shoots were rooted on half strength MS medium supplemented with 2 mg/l IBA with 100% rooting and 85% of the regenerated plantlets survived before been transferred to field conditions.

 

Key words: Medicinal plant, nodal explants, shoot regeneration, Tetrastigma hemsleyanum. 

Abbreviation

 BA, 6-Benzyladenine; GA3, gibberellic acid; IAA, indole-3-acetic acid; IBA, indole-3-butyric acid; Kn, kinetin; NAA, α-naphthaleneacetic acid; MS,Murashige and Skoog medium; 2,4-D, 2,4-dichlorophenoxyacetic acid. 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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