Abstract

The key enzyme of puerarin biosynthesis in Pueraria lobata (Willd.) Ohwi was unclear but may involve glycosylation. To investigate the regulation of puerarin biosynthesis, a putative UDP-dependent glycosyltransferase (UGT) gene, PlUGT1 was isolated from P. lobata root, which contained abundant puerarin. PlUGT1encoded 480 deduced amino acid residues with a conserved UDP-glucose-binding domain, which has 61 to 84% similarity to homologues from other plant species. SDS polyacrylamide gel electrophoresis and western blotting results showed that, fusion protein migrated as a single protein band with a molecular weight of 55 kDa. A yeast expression vector pPICZA-PlUGT1 was constructed and was transformed into Pichia pastoris strain GS115. Several recombinants containing multi-copy expression cassettes were obtained on the zeocin-YPD plate and confirmed by southern dot blotting. The yield of PlUGT1 attained 0.05 g/l when recombinant cells were cultured at pH 5.5, 30°C and induced with 0.5% methanol for 72 h. The expression of PlUGT1 protein correlates positively with the copy numbers of PlUGT1 in transformed yeast cells. These results suggest that, the PlUGT1 protein can be expressed efficiently in the P. pastoris expression system and may supply a new economic and convenient way for the production of PlUGT1 protein.

Key words: Pueraria lobata (Willd.) Ohwi, glycosyltransferase, cloning, expression,Pichia pastoris.

Abbreviation

Abbreviations: UGT, UDP-dependent glycosyltransferase; PSPG, plant secondary product glycosyltransferase; HC, high copies; ECL,electrochemiluminescence; SDS-PAGE, SDS polyacrylamide gel electrophoresis;PCR, polymerase chain reaction; RT-PCR, reverse transcription polymerase chain reaction (RT-PCR); RACE, rapid amplification of cDNA ends.