Abstract

This study determined the partial coding sequence of glucose-6-phosphate dehydrogenase (G6PD) cDNA from the Arabian camel Camelus dromedarius using reverse transcription polymerase chain reaction (RT-PCR).  Degenerate PCR primers were designed from the available data base deposited in the GenBank. Applying these primers to cDNA prepared from isolated and purified total RNA from Arabian camel liver led to the generation of two PCR products corresponding to 969 and 477 bp. These PCR products were cloned on pGEM-T-Easy cloning vector separately. Sequencing of the PCR generated cDNA fragments indicated that the 969 bp fragment represents partial coding sequence of camel G6PD and showed 93, 91 and 86% similarity with the Homo sapiens, Bos taurus and Rattus norvegicus G6PD, respectively. The Camelus dromedaries G6PD partial coding sequence encodes an open reading frame of a truncated protein consists of 323 amino acid residues that showed the conserved substrate binding site and the dinuclotide-binding fingerprint.

Key words: Arabian camel, degenerate primers, glucose 6-phosphate dehydrogenase, reactive oxygen species (ROS).