African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

A simple and rapid plate assay for screening of inulin-degrading microorganisms using Lugol’s iodine solution

Ai-Xia Li1#, Li-Zhong Guo1#, Qiang Fu2 and Wei-Dong Lu1*   #These authors contributed equally to this work.
1Shandong Provincial Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, 266109, Qingdao, People's Republic of China. 2College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, People's Republic of China.
Email: [email protected]

  •  Accepted: 27 July 2011
  •  Published: 22 August 2011

Abstract

In this report, a simple and rapid agar plate assay was established for screening of halophilic, inulin-degrading microorganisms. Two strains considered inulinolytic with this method were chosen and the inulinolytic activities in their culture supernatant were measured with the Somogyi-Nelson method, while their hydrolysis products of inulin were detected with TLC chromatogram.

 

Key words: Screening, halophilic microorganism, inulinase, Lugol’s iodine solution.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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