African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

In vitro propagation of an endangered medicinal timber species Khaya grandifoliola C. Dc.

Okere, A. U.1* and Adegeye, A.2
1National Centre for Genetic Resources and Biotechnology (NACGRAB), Moor Plantation, Ibadan, Oyo State, Nigeria. 2Department of Forest Resources Management, University of Ibadan, Ibadan, Oyo State, Nigeria.
Email: [email protected]

  •  Accepted: 14 March 2011
  •  Published: 25 April 2011

Abstract

The over exploitation of African mahogany in tropical forest has threatened the genetic base of this useful timber and medicinal tree species and as such, an experiment was conducted on the in vitro culture of Khaya grandifoliola, an endangered tree species commonly found in the high forest zones of West Africa to explore its potential for micropropagation. Embryos excised from matured seeds were cultured on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), benzyl amino purine (BAP) and kinetine (KIN) at different concentrations. The optimum result in relation to shoot length, root length, number of nodes and number of root was obtained on MS medium supplemented with 1.0 mg/L BAP + 0.01 mg/L NAA.

 

Key words: Khaya grandifoliola, African mahogany, in vitro culture, medium, embryo, plantlets.

Abbreviation

 BAP, Benzylaminopurine; KIN, kinetin; NAA, naphthalene acetic acid.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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