African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Analysis of chemical constituents in medicinal plants of selected districts of Pakhtoonkhwa, Pakistan

Iqbal Hussain1*, Riaz Ullah1,6 , Jehangir Khan5, Naeem Khan1 , Mohammad Zahoor4 , Naseem Ullah3, Muneeb ur Rehman Khattak1, Farhat Ali Khan3, Abdul Baseer3 and Muhammad Khurram3
1Department of Chemistry, Kohat University of Science & Technology, KPK, Pakistan. 2Institute of Chemical Sciences, University of Peshawar, KPK, Pakistan. 3Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, KPK, Pakistan. 4Department of Chemistry, University of Malakand, Malakand, KPK, Pakistan. 5Department of Pharmacy, University of Malakand, Malakand, KPK, Pakistan. 6Department of Chemical Engineering, College of Engineering, King Saud University, P.O BOX 800, Riyadh 11421, Saudi Aarbia.
Email: [email protected]

  •  Accepted: 15 September 2011
  •  Published: 10 October 2011

Abstract

Alkaloids, flavonoids and saponins were determined in the Teraxacam officinale,Cichorium intybus and Figonia tritica. Quantative determinations of crude alkaloids, flavonoids and saponins (g kg-1) and their percentages (%) were determined in C. intybusT. officinale and F. critica collected from Kohat, Mardan, Nowshera and Peshawar regions. Thin layer chromatography (TLC) study of the alkaloids, flavonoids and saponins were carried out with different solvent systems and color of the spot and Rf value of each constituent was determined. Among the samples ofC. intybus, relatively higher contents of alkaloids (14 g kg-1) were found in the sample collected from Mardan region, followed by the sample gathered from Nowshera which is equal to 13.8g kg-1. Higher contents of flavonoids were found inC. intybus collected from Kohat region which was 23.48 g kg-1. The other remaining samples of C. intybus ranged in the decreasing order of 19.7, 17.2 and 10.0 g kg-1for the ones collected from Nowshera, Mardan and Peshawar, respectively. The overall saponin contents remained almost low as compared to the alkaloid and flavonoid contents.

 

Key words: Alkaloids, flavonoids, saponins, TLC study, medicinal plants.