African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12482

Full Length Research Paper

Amplified fragment length polymorphism (AFLP) studies on Indian Cycas species

  P. Radha1* and Rita Singh2
  1Centre for Plant Molecular Biology, Osmania University, Hyderabad 500 007, Andhra Pradesh, India. 2University School of Environment Management, Guru Gobind Singh Indraprastha University, New Delhi-100 075, India.
Email: [email protected]

  •  Accepted: 15 April 2011
  •  Published: 11 July 2011

Abstract

 

Amplified fragment length polymorphism (AFLP) technology was used to reveal the genetic variation in six species of Cycas collected from eleven natural populations. Two sets of primer with 4-selective nucleotides were used in this study and 78% polymorphism was found. The results correlated with geographical distribution of the plants and grouped into four. This study is highly useful in the delimitation of Indian Cycas species which has been enunciated as highly endangered taxa by IUCN Red Data List.

 

Key words: Cycads, Cycas, amplified fragment length polymorphism (AFLP), polymorphism.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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