African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Toxicity bioassay of lead acetate and effects of its sub-lethal exposure on growth, haematological parameters and reproduction in Clarias gariepinus

Hmoud Fares Alkahemal-Balawi, Zubair Ahmad*, Ali Suliman Al-Akel, Fahad Al-Misned, El-Amin Mohamad Suliman and Khalid Abdullah Al-Ghanim
Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia.
Email: [email protected], [email protected]

  •  Accepted: 18 August 2011
  •  Published: 14 September 2011

Abstract

 

The African catfish, Clarias gariepinus, was exposed to different concentrations of lead acetate for 96 h, and the value of LC50 for the exposure period was registered as 122 mg/L. The effects of sub-lethal exposure on growth, reproduction and haematological parameters were also investigated in C. gariepinus. Lead exposure at all concentrations reduced growth rate, and was inversely related to growth. There were significant effects of lead exposure on erythrocyte count, haemoglobin concentration and haematocrit values. Lead exposure time and dose increased the plasma glutamic-oxaloacetic transaminase (PGOT) and plasma glutamic-pyruvic transaminase (PGPT) activity levels. No significant effect of lead acetate was noticed on fecundity and ovum size. Sperm motility was reduced in all concentrations of lead acetate after 4 weeks of exposure. The present investigation indicates that the lead acetate is less toxic to fish than other metals as the LC50value is comparatively high.

 

Key words: Lead acetate, acute toxicity, growth, haematological parameters, reproduction.