Full Length Research Paper
Abstract
In the regeneration of Acacia nilotica, the effect of cytokinin (BA) and auxin (IBA) in factorial experimentation on the MS medium was determined with different dosages each. Starting from the epicotyls of germinated seeds, a combination of benzylaminopurin, naphtaleneacetic acid and gibberelic acid (BA*NAA*GA3) served as a good initiator of adventitious buds. However, the best production of nodes was obtained with BA for 0.1 mgl-1 dosage. The rate of node production was equal to 6.767%, while the longest stem was 53.33 mm. The best and high rate of rooting was 93.33 and 83.33%, respectively with 0.1 and 1.0 mg/l of IBA. The interaction between the two hormones had a negative effect on the growth and rooting of the shoots, whilst high dosage had a shortening effect on the nodes.
Key words: Acacia nilotica, regeneration, microcutting, rooting, growth, cotyledonary node.
Abbreviation
BA, Benzylaminopurin; IBA, indol-3-butyric acid; NAA, naphtalenacetic acid; GA, gibberelic acid.
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