African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Spent bleaching clay (SBC) from oil refining as a substrate for the spawn production of shiitake mushroom (Lentinula edodes)

  Xiqin Wang1,2, Jianqiang Zhang1, Benhong Liu3* and Dunlian Qiu4,  
1School of Environmental Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. 2College of Rural and Urban Construction, Chengdu University, Chengdu 610106, China. 3College of Architecture and Environment, Sichuan University, Chengdu 610065, China. 4Chengdu Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China.
Email: [email protected]

  •  Accepted: 12 November 2010
  •  Published: 27 December 2010

Abstract

 

While no effective (both in a technical and economic sense) methods exist so far to handle large quantities of untreated spent bleaching clay (SBC) in China, there are indeed great demands for low-cost alternatives for mushroom substrate. Hence, it is in the interest of both edible-oil-refining industries and mushroom producers to explore how partially substituting traditional shiitake substrate by SBC would influence the mycelial growth in shiitake spawn production. This paper conducted a comparative shiitake spawn production experiment with a conventional shiitake substrate formula comprising of 78% sawdust, 20% wheat bran, 1% magnesium sulfate, and 1% gypsum (percentage by weight, the same thereafter) as the control and substrate formulae with the substitution of SBC for part of sawdust or wheat bran as treatments to observe the mycelial growth. The results indicated that formulae with SBC had lower incidence of microbial contamination and higher mycelial growth speed, and the optimal formula was 15% SBC, 78% coarse sawdust, 5% wheat bran, 1% magnesium sulfate and 1% gypsum. Therefore, the utilization of SBC in shiitatke spawn production not only considerably reduces the costs of shiitake production but also bring about substantial environmental benefits.

 

Key words: Spent bleaching clay, shiitake mushroom, Lentinula edodes, vegetable oils, spawn production, mycelial growth, components.