African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular diagnosis of Wolbachia endosymbiont from Iranian scorpion Hemiscorpius lepturus using polymerase chain reaction (PCR) amplification of 16S rDNA gene

Masoumeh Baradaran3, Amir Jalali1,3*, and Abbas Jolodar2
1Department of Pharmacology and Toxicology, School of Pharmacy, Jundishapur University of Medical Sciences, Ahwaz, Iran. 2Department of Basic Sciences, Faculty of Veterinary Medicine, ShahidChamran University of Ahvaz, Ahvaz, Iran. 3Toxicology Research Center, Jundishapur University of Medical Sciences, Ahwaz, Iran.
Email: [email protected]

  •  Accepted: 19 October 2011
  •  Published: 28 December 2011

Abstract

There are several reports concerning the intracellular bacteria, Wolbachia, which infect an extensive range of invertebrates and promote a diverse array of reproductive alteration which encourage infection spread among its host population. This study reports the identification of Wolbachia in the Iranian scorpion namedHemiscorpius lepturus (H. lepturus) collected from the southwestern province of Khuzestan using partial polymerase chain reaction (PCR) amplification of Wolbachia16S rDNA gene. PCR product was directly sequenced and the alignment of the sequence with similar sequences in GenBank showed high similarity with 16S rDNA gene of Wolbachia endosymbiont of Drosophila melanogaster.

 

Key words: Wolbachia, Iranian scorpion, 16S rDNA gene, Hemiscorpius lepturus.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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