African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Induction and optimization of cellulases using various agro-wastes by Trichoderma virdii: Effect of alkali pretreatment

Meenakshi Goyal*
  • Meenakshi Goyal*
  • Department of Biochemistry, Punjab Agricultural University, Ludhiana 141001, India.
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Giridhar Soni
  • Giridhar Soni
  • Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141001, India
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  •  Received: 25 February 2014
  •  Accepted: 28 July 2014
  •  Published: 13 August 2014

Abstract

This study presents optimization of various lignocellulosics and alkali pretreatment for maximum cellulase production by Trichoderma virdii sp. Maximum endoglucanase (642 IU/L) and exoglucanase (187IU/L) activity was achieved with maize straw at 5% concentration. Oat hay was the most suitable agro-waste for β - glucosidase (7100 IUL-1) production followed by maize straw (6500 IUL-1). Maize straw was chosen in an effort to enhance cellulase production with 0.1 N NaOH, 0.5 N NaOH and I.0 N NaOH pretreatment. 0.1 N NaOH produced desirable results showing 2.5, 1.6, and 1.7 fold increase in endoglucanase, exoglucanase and β-glucosidase activity. This 0.1 N pretreated straw produced reducing sugars 3.5 times more than untreated straw.

 

Key words: Lignocellulosics, maize straw, cellulase production, Trichoderma virdii, alkali pretreatment

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