African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

Selection and evaluation of Rhizobial strains of Vigna radiata L. beneficial to biological nitrogen fixation

Neeraj1*, Gaurav S.S.1, Sachin2 and Chandra M3
1Department of Biotechnology, C.C.S. University, Meerut-250110 (U.P) India. 2Division-FLOF, Central Institute of Medicinal and Aromatic Plants, Lucknow- 226015 (U.P), India. 3Scientific and Applied Research Center, Meerut. U.P. INDIA
Email: [email protected]

  •  Accepted: 15 August 2008
  •  Published: 20 October 2008

Abstract

This study aimed to select suitable strains that can be used as inoculants to enhance legume production and simultaneously reduce the use of inorganic fertilizers.  Evaluations of the selected strains of Vigna radiata on the basis of polysaccharide (exopolysaccharides and lipopolysaccharides) produced reinforced evidence that Rhizobial strains and its mutant nodulate promote growth differently under stressed environments. Strains performance evaluation demonstrated that the R0132 (1106):Tn5 inoculant of Sinorhizobium fredii (Formerly, Rhizobium fredii)is a prime candidate as a commercial inoculant. It benefited growth of V. radiataand it was more easily cultured on solid and liquid media than any of the other strains tested including R0132 (1112):Tn5, R0132 (0097):Tn5 and R0132 (1106):Tn5. Mutant R0132 (1106):Tn5 exhibited superior growth promoting ability under extreme environmental condition; therefore it has potential to be used in India.

 

Key words: Rhizobial mutant, biological N2 fixation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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