African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Determination of total polyphenol content and antityrosinase capacity of mulberrymedicine (Morusnigra L.) extract

Wu Chun, Xu Li*, Wang Yuancheng, Chen Hu and Huang Xianzhi
College of Biotechnology, Southwest University, Chongqing 400716, China.
Email: [email protected]

  •  Accepted: 05 October 2011
  •  Published: 30 November 2011

Abstract

Total polyphenol content and antityrosinase capacity of mulberry medicine extract were evaluated for its development and utilization. Total polyphenols were extracted from mulberry medicine with 70% alcohol solution. The content of total polyphenols was determined by ultraviolet spectrophotometer and the capacity of antityrosinase was evaluated by using the enzyme-linked immunosorbent assay (ELISA) method. Results show that the content of polyphenols was up to 8.668 mg/g and tyrosinase half-inhibitory concentration (IC50) was 12.12 mg/ml. Kinetic study indicated that the mechanism of tyrosinase inhibition was mixed type inhibition.

 

Key words: Morusnigra L., extraction of medicine mulberry, polyphenols, tyrosinase.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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