African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Effect of two follicle stimulating hormone (FSH) preparations and simplified superovulatory treatments on superovulatory response in Xinji fine-wool sheep

Weiwei Wu1,2, Hanikezi2, Mei Yang3, Ping Gong2, Feng Wang1, Yuezhen Tian2, Xinming Xu2, Xuefeng Fu2, Haqikezi2, Kechuan Tian2* and Zhiqin Guo2*
1Center of Animal Embryo Engineering and Technology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. 2Xinjiang Academy of Animal Science, Urumqi 830000, China. 3Dalian Administration of Animal Health by Supervision, Dalian.116000, China
Email: [email protected] or [email protected]

  •  Accepted: 02 August 2011
  •  Published: 30 November 2011

Abstract

The objective of the present study was to investigate the response of Xinji fine-wool sheep to superovulation with two follicles stimulating hormone (FSH) preparations and simplified superovulatory treatments. In Experiment I, 22 adult Xinji fine-wool sheep were randomly allocated in equal number (n = 11) to two groups which were superovulated with 280 IU FSH-P and 150 mg Folltropin-V, respectively. In Experiment II, 61 adult Xinji fine-wool sheep randomly divided into four groups were treated with four different superovulatory schemes based on the dosage and number of Folltropin-V injection, respectively. The ewes in the four groups were treated with a total of 150 mg Folltropin-v (6 injection, Group A, n = 15), 120 mg Folltropin-V (3 injection, Group B, n = 18), 90 mg Folltropin-V (3 injection, Group C, n = 13) and 150 mg Folltropin-V dissolved in 30% PVP (1 injection, Group D, n = 15), respectively. The results show that the number of corpora lutea was significantly higher in ewes treated with Folltropin-V than that with FSH-P (P<0.05); higher number of transferable embryos was observed in Folltropin-V group than in FSH-P group. There is no significant difference in number of recovered embryos and transferable embryos in Groups A and B (P>0.05). However, the number of recovered embryos and transferable embryos in Groups C and D is significantly lower than those in Groups A and B (P<0.05). In conclusion, superovulation with 120 mg Folltropin-V (3 injections) is the most appropriate superovulatory treatment in Xinji fine-wool sheep.

 

Key words: Superovulatory response, follicle stimulating hormone (FSH) preparations, Xinji fine-wool sheep.