African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic diversity and population structure among isolates of the brown spot fungus, Bipolaris oryzae, as revealed by inter-simple sequence repeats (ISSR)

Basavaraju Archana
  • Basavaraju Archana
  • Department of Studies in Biotechnology, Asian Seed Health Centre, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India
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Kukandhuru R. Kini
  • Kukandhuru R. Kini
  • Department of Studies in Biotechnology, Asian Seed Health Centre, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India
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Harishchandra S. Prakash
  • Harishchandra S. Prakash
  • Department of Studies in Biotechnology, Asian Seed Health Centre, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India
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  •  Received: 24 January 2013
  •  Accepted: 23 October 2013
  •  Published: 08 January 2014

Abstract

Bipolaris oryzae, the rice brown spot fungus is one of the pathological threats to rice crop worldwide. The genetic diversity among the Indian isolates of brown spot pathogen was studied using inter-simple sequence repeats (ISSR). Considerable intraspecific variability among the isolates of B. oryzae was revealed. Eighty-seven (87) highly reproducible fragments were amplified in all 69 isolates with an average of 13.8 markers per primer. Polymorphism range shown by ISSR primers was 83.33 to 95.45%, while the range of total loci scored was from 6 to 22. The molecular weight of scorable loci ranged from 150 to 2600 bp. The results obtained confirmed the genetic diversity of rice brown spot fungus among samples under study.  The present study suggests that ISSR markers can be used as robust molecular markers for the population genetics, epidemiological and ecological studies of B. oryzae that will aid in designing improved methods for management of the rice brown spot disease.

 

Key words: Bipolaris oryzae, rice brown spot, inter-simple sequence repeats (ISSR), dendrogram, polymorphism.

Abbreviation

Abbreviations: RFLP, Restriction fragment length polymorphism; RAPD, random amplified polymorphic DNA; AFLP, amplified fragment length polymorphic DNA; VNTR, variable number tandem repeats; ISSR, inter-simple sequence repeats; PDA, potato dextrose agar; PDB, potato dextrose broth.

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