African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Biological hardening and genetic fidelity testing of micro-cloned progeny of Chlorophytum borivilianum Sant. et Fernand

Archana Mathur, Ajay Kumar Mathur*, Priyanka Verma, Shrawan Yadav, Moti Lal Gupta and Mahendra P. Darokar
Central Institute of Medicinal and Aromatic Plants (CIMAP) PO CIMAP; Picnic Spot Road; Lucknow –226015 India.
Email: [email protected] or [email protected]

  •  Accepted: 25 February 2008
  •  Published: 17 April 2008

Abstract

 

The micro-cloned plantlets of Chlorophytum borivilianum registered more than 95% establishment in soil following treatment with various bio-inoculants namely;Glomus aggregatum, Trichoderma harazianum and Piriformospora indicawhereas Azospirullum sp. (CIM-azo) and Actinomycetes sp. (CIM-actin) showed only up to 85% plantlet establishment. The un-rooted shoots were also treated with these bio-inoculants, for in vivo root induction and increased survival rate/establishment frequency when transferred to soil. The un-rooted shoots also showed in vivo rooting (50%) when treated with mycorrhiza Glomus aggregatum(VAM) and Trichoderma harazianum. The genetic fidelity testing of micro-cloned, bio-hardened progeny based on a RAPD analysis using 40 random decamer DNA primers indicated a strong uniformity in relation to the parent genotype.

 

Key words: Chlorophytum borivilianum, bio-inoculants, bio-hardening, genetic fidelity, RAPD analysis.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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