African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis

H S Rattanpal*1, Gagandeep Kaur2 and Monika Gupta2
1Department of Horticulture, Punjab Agricultural University, Ludhiana 141004, Punjab, India. 2School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141004, Punjab, India.
Email: [email protected]

  •  Accepted: 04 May 2011
  •  Published: 17 October 2011

Abstract

This study was aimed to optimize the organogenesis in rough lemon (Citrus jambhiri Lush), an important citrus rootstock of India. Organogenesis was induced in epicotyl segments of rough lemon seedlings. Three important factors influencing organogenesis in vitro viz. hormone combination, cut modes and photoperiod were evaluated. Etiolation of seedlings had a positive effect on bud formation and higher number of buds per explant was obtained from etiolated seedlings. Among the cut modes, transverse cut performed better for its effect on the number of regenerated buds and shoots per explant. The best response regarding the number of adventitious buds formed per explant was observed with BAP at the concentration of 2.0 mg/l along with malt extract (500 ppm) and additional dose of 25 g/l sucrose.

 

Key words: Citrus, epicotyl, hypocotyl, etiolation, transverse cut, longitudinal cut, photoperiod.

Abbreviation

BAP, Benzyl amino purine; NAA, Napthaline acetic acid;  IBA,Indoline butyric acid

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 10 No. 63
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES