Abstract

Olive, being a fruit of subtropical climate and recognized to have significant importance in terms of human health, is being investigated with different aspects. Wild olives are valuable in the sense that they contain the sources of stability and they can be separated by using random amplified polymorphic DNA (RAPD) technique. In this study, a total of 59 primers were used for the purpose of determining the DNA fingerprints of 12 wild olives obtained from different provinces of Aegean region, and 92 polymorphic bands were yielded. Mean number of polymorphic bands per individual was calculated as 7.67 and number of polymorphic bands per scorable primer was calculated as 4.6. Specific DNA markers are important for determining the genetic relation among wild subspecies in olives. The purpose of this study is to determine the optimum conditions for genomic DNA in identifying the wild olives by using RAPD analysis, and to perform primer trials. In conclusion, the genetic relation between wild olives were determined through Jaccard, Sorensen Dice, Simple Matching coefficients, (unweighted pair group method with arithmetic averages) UPGMA and neighbor joining method by using the data obtained with RAPD.

Key words: Random amplified polymorphic DNA (RAPD), Olea europaea, arbitrary primers, Jaccard, Sorensen Dice, Simple Matching, unweighted pair group method with arithmetic averages, neighbor joining.

Abbreviation

Abbreviations: RAPD, Random amplified polymorphic DNA; CTAB, cetyl trimethyl ammonium bromide; EDTA, ethylene diamine tetraacetic acid; UPGMA,unweighted pair group method with arithmetic averages; NJ, neighbor joining.