African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12483

Full Length Research Paper

The use of microsatellites in the characterization of three plantain cultivars from the Democratic Republic of Congo

Mave Dhed’asi Gracia
  • Mave Dhed’asi Gracia
  • Department of Biotechnology, University of Kisangani, P. O. Box 2012 Kisangani, Democratic Republic of Congo.
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Dhed’a Djailo Benoit
  • Dhed’a Djailo Benoit
  • Department of Biotechnology, University of Kisangani, P. O. Box 2012 Kisangani, Democratic Republic of Congo.
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Kateregga John N.
  • Kateregga John N.
  • College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062 Kampala, Uganda.
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Nanyiti Sarah
  • Nanyiti Sarah
  • National Crop Resources Research Institute, Namulonge Uganda, P. O. Box 7084 Kampala, Uganda.
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  •  Received: 10 February 2023
  •  Accepted: 18 May 2023
  •  Published: 30 June 2023

Abstract

Knowledge of the genetic variability of plantain cultivars in the Democratic Republic of the Congo (DRC) is limited. This study assessed genetic diversity and relationships in three plantain accessions differing in pseudostem color at seven microsatellite loci using SSR markers. DNA was extracted from banana leaves, and PCR was performed with unlabeled and fluorescent-labeled SSR primers and eventual capillary sequencing. Different population genetic analysis software was used to interpret the fragment analysis data. There was a high allelic power with a mean polymorphic information content of 0.83. A high average genetic diversity was indicated by Nei's Gene Diversity Index (h: 1.38) and the Shannon Information Index (I: 2.22). Pairwise genetic similarity between plantain genotypes averaged 0.82. A dendrogram grouped the 60 genotypes into three clusters. AMOVA showed high genetic variation within the population (98%) compared to the variation between populations, with no significant difference between the three cultivars regarding the color of the pseudostem. In contrast, these cultivars were closely related to each other. No correlation between the morphological and molecular characterization of cultivars was observed. We recommend a follow-up study with other plantain cultivars from other regions and large sample size for more representative data.

 

Key words: Characterization, microsatellites, genetic diversity, plantain cultivars.