African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

Full Length Research Paper

Multi-resistance to carbapenems by the production of Imipenemase (IMP)-types carbapenemases in Gram-negative bacilli in Burkina Faso

Damis Yves Patrik Bouniounou
  • Damis Yves Patrik Bouniounou
  • Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
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Amana Metuor Dabire
  • Amana Metuor Dabire
  • Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
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Rahimatou Yasmine Tiemtore
  • Rahimatou Yasmine Tiemtore
  • Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
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Serge Sougue
  • Serge Sougue
  • Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
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Pegdwende Rose Bonkoungou
  • Pegdwende Rose Bonkoungou
  • Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
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Jacques Simpore
  • Jacques Simpore
  • Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
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  •  Received: 07 November 2023
  •  Accepted: 05 December 2023
  •  Published: 29 February 2024

Abstract

The growing prevalence of Imipenemase (IMP) metallo-beta-lactamases (MBL) producing strains of Gram-negative bacilli (GNB) pathogens is a real concern for clinicians in the light of therapeutic impasses driven. However, resistance genes encoding these enzymes are hardly documented in Burkina Faso. This study aims to show carbapenem-resistance mediated by the production of IMP-type carbapenemase in GNB clinical strains collected in Ouagadougou, Burkina Faso. Strains resistance profile to imipenem, meropenem, ertapenem, doripenem and aztreonam was determined by the disk diffusion method. Classical polymerase chain reaction (PCR) was carried out to detect β-lactamase (bla) gene of IMP in resistant strains. Out of 158 GNB collected, 91 (57.6%) were resistant to at least one of the carbapenems and/or to aztreonam. The highest prevalence of resistant strains was observed in Escherichia coli (45.1%; n=41) and Klebsiella pneumoniae (26.5% n=24). Among 32 resistant strains blaIMP gene positive (35.2%), Escherichia coli was the predominant species carrying resistance gene (18.7%, n=17/91). The findings strengthen the scarce existing scientific data on antimicrobial resistance mediated by metallo-beta-lactamases (MBL) in Burkina Faso.

 

Key words: Carbapenem resistance, carbapenemase, blaIMP, Gram-negative bacilli, Burkina Faso.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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